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Which of the following is the best way to make many copies of a vector containing recombinant DNA?


A) polymerase chain reaction
B) DNA hybridization
C) reproductive cloning
D) DNA cloning

E) A) and C)
F) All of the above

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During ________ cloning,the nucleus of an egg cell is replaced with the nucleus of a somatic cell from the individual being cloned.

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The induction of pluripotent stem cells requires that specific gene or proteins be added to a differentiated cell.

A) True
B) False

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True

Suspect DNA is isolated from a murder scene.The DNA is used to create a DNA fingerprint for the suspect,but it does not match the DNA fingerprint of any of the known suspects,and it does not have a match in CODIS.The murderer is not identified and the crime remains unsolved.Although there are no matches,forensic officers enter the murder scene DNA fingerprint into CODIS because


A) it may exonerate someone who was wrongly imprisoned for another crime.
B) a match may be entered into CODIS at a later date, solving the case.
C) it may be used in a paternity suit.
D) making it public will help civilians solve the case.

E) None of the above
F) All of the above

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Stem cells that can generate all body cell types,including those in the birth sac,are described as ________.

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Which of the following is NOT involved in creating recombinant DNA?


A) restriction enzymes
B) ligase
C) RNA
D) plasmids

E) B) and D)
F) B) and C)

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A scientist isolates genomic DNA from a human cell.She needs to physically separate a specific segment of that DNA away from the rest.Which technology will allow her to do this?


A) DNA microarrays
B) genetic engineering
C) restriction enzymes
D) recombinant DNA

E) A) and B)
F) A) and C)

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In RNA ________,the expression of a specific gene is blocked by small pieces of RNA.

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Genetically modified plants can pass their recombinant DNA to wild plants of the same species through their pollen.

A) True
B) False

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Crops genetically engineered to be resistant to an herbicide might promote an increased use of that herbicide because


A) the herbicide is now an effective way to prevent weed growth without harming the crop plant.
B) the engineered crops will transfer the herbicide resistance to wild plants.
C) more farmers will be able to afford the genetically engineered seeds and so more of the crop will be planted.
D) a higher concentration of the herbicide will be needed to kill wild plant species.

E) A) and B)
F) All of the above

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Genetically modified organisms may contain proteins,DNA,and ________ from another organism.

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To screen the genome of a bacterium for a particular gene,you would use ________that attach to complementary DNA sequences.


A) gels
B) ligases
C) DNA probes
D) restriction enzymes

E) B) and D)
F) B) and C)

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Potatoes have been engineered to produce proteins from the bacteria that causes cholera,a diarrheal disease.This is an example of genetic engineering to


A) enhance economically important species.
B) produce pharmaceutical products.
C) produce vaccines.
D) produce dangerous organisms.

E) A) and D)
F) A) and C)

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RNA interference can only be used to treat genetic diseases.

A) True
B) False

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DNA polymerase is used in the laboratory to


A) perform gel electrophoresis procedures.
B) cure patients of certain genetic diseases.
C) make many copies of a DNA sequence.
D) attach DNA fragments to each other.

E) A) and B)
F) B) and C)

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C

In research,restriction enzymes are used to


A) stop replication of DNA at a specific base during DNA sequencing.
B) cut DNA molecules into pieces that can be used to create recombinant DNA.
C) measure the relative amounts of each base in a DNA molecule.
D) join two fragments of DNA together to form a molecule of recombinant DNA.

E) All of the above
F) A) and B)

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You are shown the following result of gel electrophoresis.Column 1 represents blood found on the arm of a crime victim,but it is not the victim's blood.The other columns are blood taken from various suspects.Which suspect's blood was found on the victim's arm? You are shown the following result of gel electrophoresis.Column 1 represents blood found on the arm of a crime victim,but it is not the victim's blood.The other columns are blood taken from various suspects.Which suspect's blood was found on the victim's arm?   A)  2 B)  3 C)  4 D)  5


A) 2
B) 3
C) 4
D) 5

E) B) and C)
F) None of the above

Correct Answer

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Why are housekeeping genes not used to create DNA fingerprints?


A) They mutate often because they are unnecessary genes and so are highly variable.
B) They are important genes for every cell in every person and so are highly conserved.
C) They are found only in a few cell types, not all cell types.
D) They are expressed at a low rate in cells.

E) All of the above
F) None of the above

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B

A restriction enzyme from a bacterium is able to cut a human's DNA because


A) bacteria and humans are both prokaryotes.
B) all organisms use the same DNA nucleotides.
C) bacteria and humans have identical genomes.
D) a given restriction enzyme can cut DNA at several different sequences.

E) A) and B)
F) B) and D)

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It is theoretically impossible for two individuals (other than identical twins)to have the same DNA fingerprint.

A) True
B) False

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