Question 1

Which of the following is essential in PCR?

Accepted Answer

A)   DNA polymerase
B)   antisense RNAs
C)   reverse transcriptase
D)   DNA primers
E)   both DNA primers and DNA polymeraseF) A) and B)
G) A) and D)

Question 2

A technique using fluorescent-tagged probes to detect specific DNA sequences in their natural locations is known as (FISH/PCR/BLOTTING).

Accepted Answer

The answer of A technique using fluorescent-tagged probes to detect...

Question 3

Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a sample?

Accepted Answer

A)   microarray
B)   cloning
C)   PCR
D)   genome mapping
E)   microarray or PCRF) C) and D)
G) B) and D)

Question 4

________ are used in the detection of DNA by FISH.

Accepted Answer

A)   Restriction fragments
B)   Reverse transcriptase and DNA polymerase
C)   Synthetic DNAs and fluorescent tags
D)   Compressed air and gold beads
E)   DNA polymerase and DNA ligaseF) A) and D)
G) None of the above

Question 5

The templates for next-generation sequencing are

Accepted Answer

A)   double-strand restriction fragments.
B)   double-strand DNA on silicon chips.
C)   double-strand PCR products.
D)   single-strand DNA on silicon chips.
E)   single-strand DNA on slides.F) All of the above
G) B) and E)

Question 6

Restriction enzymes are useful only on synthetic DNAs.

Accepted Answer

A) True 
 B)False  False

Question 7

Which of the following microbes produces a protein that kills a variety of insect pests?

Accepted Answer

A)   Pseudomonas
B)   Haemophilus influenzae
C)   Bacillus thuringiensis
D)   Thermus aquaticus
E)   Plasmodium falciparumF) B) and E)
G) B) and D)

Question 8

If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure,what would be the result?

Accepted Answer

A)   DNA replication would occur more slowly than normal.
B)   DNA replication would not occur at all.
C)   Many mistakes would occur.
D)   DNA replication would occur twice as fast as normal.
E)   DNA replication would stop after one cycle.F) A) and E)
G) A) and B) E

Question 9

Sticky-end fragments generated by EcoRI will hydrogen bond to any other sticky-end sequence.

Accepted Answer

A) True 
 B)False

Question 10

Some of the strongest opposition to the application of recombinant DNA technology concerns

Accepted Answer

A)   DNA fingerprinting.
B)   modification of food crops.
C)   correcting gene defects in animals.
D)   sequencing of the human genome.
E)   pest control measures.F) C) and D)
G) A) and E) B

Question 11

Which of the following microbes has been genetically engineered to contain a protein that helps protect crops from freezing?

Accepted Answer

A)   Deinococcus radiodurans
B)   Bacillus thuringiensis
C)   Pseudomonas
D)   Salmonella
E)   Escherichia coliF) A) and B)
G) C) and D)

Question 12

Explain what a transgenic organism is,and give two examples.

Accepted Answer

A transgenic organism is one that contai...

Question 13

Which of the following is common to both Sanger sequencing and next-generation sequencing?

Accepted Answer

A)   radioactive labels
B)   gel electrophoresis
C)   tethered DNA fragments
D)   chain terminating nucleotides
E)   lasersF) B) and D)
G) B) and C)

Question 14

Gene therapy for human genetic diseases has not been successful yet.

Accepted Answer

A) True 
 B)False

Question 15

Nucleic acid molecules used to deliver new genes to cells are called (plasmids/vectors/clones).

Accepted Answer

The answer of Nucleic acid molecules used to deliver new...

Question 16

Pest resistant cotton plants that produce Bt were produced by selective breeding.

Accepted Answer

A) True 
 B)False

Question 17

Beta-carotene,the biochemical precursor to vitamin A,can be added to rice by using (biotechnology/cloning/recombinant)DNA technology,thereby increasing its nutritional value.

Accepted Answer

The answer of Beta-carotene,the biochemical precursor to vitamin A,can be...

Question 18

The natural role of restriction enzymes in bacteria is to

Accepted Answer

A)   make conjugation more efficient.
B)   allow transposons to move to another place in the chromosome.
C)   protect the cell from invading phages.
D)   allow cells to accept foreign DNA.
E)   provide the cell with new phenotypes, such as antibiotic resistance.F) A) and C)
G) C) and D)

Question 19

Which of the following items is NOT a part of the name of a restriction enzyme?

Accepted Answer

A)   the Gram reaction of the source bacterium
B)   the specific epithet of the source bacterium
C)   Roman numerals to indicate its order of discovery
D)   the strain of the source bacterium
E)   the genus of the source bacteriumF) B) and C)
G) B) and E)

Question 20

Which of the following devices is used for PCR?

Accepted Answer

A)   an electrophoresis chamber
B)   a gene gun
C)   a DNA sequencer
D)   a thermocycler
E)   a nucleic acid synthesis machineF) A) and C)
G) D) and E)

Exam 8: Recombinant DNA Technology

Which of the following is essential in PCR?

Correct AnswerverifiedShow Answer

A technique using fluorescent-tagged probes to detect specific DNA sequences in their natural locations is known as (FISH/PCR/BLOTTING).

Correct AnswerverifiedShow Answer

Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a sample?

Correct AnswerverifiedShow Answer

________ are used in the detection of DNA by FISH.

Correct AnswerverifiedShow Answer

The templates for next-generation sequencing are

Correct AnswerverifiedShow Answer

Restriction enzymes are useful only on synthetic DNAs.

Correct AnswerverifiedFalse

Which of the following microbes produces a protein that kills a variety of insect pests?

Correct AnswerverifiedShow Answer

If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure,what would be the result?

Correct AnswerverifiedE

Sticky-end fragments generated by EcoRI will hydrogen bond to any other sticky-end sequence.

Correct AnswerverifiedShow Answer

Some of the strongest opposition to the application of recombinant DNA technology concerns

Correct AnswerverifiedB

Which of the following microbes has been genetically engineered to contain a protein that helps protect crops from freezing?

Correct AnswerverifiedShow Answer

Explain what a transgenic organism is,and give two examples.

Correct AnswerverifiedA transgenic organism is one that contai...View AnswerShow Answer

Which of the following is common to both Sanger sequencing and next-generation sequencing?

Correct AnswerverifiedShow Answer

Gene therapy for human genetic diseases has not been successful yet.

Correct AnswerverifiedShow Answer

Nucleic acid molecules used to deliver new genes to cells are called (plasmids/vectors/clones).

Correct AnswerverifiedShow Answer

Pest resistant cotton plants that produce Bt were produced by selective breeding.

Correct AnswerverifiedShow Answer

Beta-carotene,the biochemical precursor to vitamin A,can be added to rice by using (biotechnology/cloning/recombinant)DNA technology,thereby increasing its nutritional value.

Correct AnswerverifiedShow Answer

The natural role of restriction enzymes in bacteria is to

Correct AnswerverifiedShow Answer

Which of the following items is NOT a part of the name of a restriction enzyme?

Correct AnswerverifiedShow Answer

Which of the following devices is used for PCR?

Correct AnswerverifiedShow Answer

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False

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E

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B

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A transgenic organism is one that contai...
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