Question 1

Intermittent sterilization, which uses 3 days of lower temperature steam for short periods of time, is also called ______.

Accepted Answer

A)   pasteurization
B)   incubation
C)   tyndallization
D)   disinfection
E)   desiccationF) A) and C)
G) All of the above

Question 2

Continued widespread use of the phenolic compound triclosan is advantageous in that it is important to remove as many organisms as possible from our hands and there is little risk of resistant organisms evolving.

Accepted Answer

A) True 
 B)False

Question 3

Endospores can be inactivated by _______.

Accepted Answer

A)   dry heat at 170°C for 2 hours
B)   incineration
C)   glutaraldehyde for 3 or more hours
D)   ethylene oxide for 3 or more hours
E)   All of the choices are correct.F) None of the above
G) A) and E)

Question 4

A method for sterilizing milk, called _____ treatment, uses 134°C for 1 to 2 seconds.

Accepted Answer

A)   pasteurization
B)   batch pasteurization
C)   ?ash pasteurization
D)   ultra high temperature
E)   tyndallizationF) B) and C)
G) A) and E)

Question 5

Alcohols _______.

Accepted Answer

A)   denature proteins when in a 50-95% alcohol-water solution
B)   disinfect items when soaking method is utilized
C)   are skin degerming agents
D)   at 50% or higher concentrations dissolve cell membrane lipids
E)   All of the choices are correct.F) All of the above
G) A) and E)

Question 6

The presence of organic matter such as saliva and pus can interfere with the actions of
disinfectants.

Accepted Answer

A) True 
 B)False

Question 7

Chlorine and ethylene oxide are sterilizing gases.

Accepted Answer

A) True 
 B)False

Question 8

Surfactants work by _______.

Accepted Answer

A)   coating the organism preventing interaction with its environment
B)   blocking transport into the organism
C)   blocking transport out from the organism
D)   disrupting membrane integrityE) B) and D)
F) A) and D)

Question 9

When a control agent targets the metabolic processes of microbial cells, active younger cells
typically die more rapidly than older cells.

Accepted Answer

A) True 
 B)False

Question 10

Organisms in the genus Pseudomonas are resistant to quats.

Accepted Answer

A) True 
 B)False

Question 11

_____ is a halogen used in gaseous and liquid form for large scale disinfection of drinking water and sewage.

Accepted Answer

A)   Iodine
B)   Chlorine
C)   Bromine
D)   Fluorine
E)   BetadineF) A) and B)
G) B) and E)

Question 12

Which of the following represents microbial control by osmotic pressure?

Accepted Answer

A)   Canning tomatoes after a summer growing season to use throughout the winter
B)   The crew of the Mayﬂower using salted meats throughout their voyage to the New World
C)   Placing fruit slices on a drying rack
D)   Pickling cucumbers and other vegetables for long-term storageE) C) and D)
F) A) and B)

Question 13

The method of removing vegetative microbial life forms and debris to reduce contamination to safe levels is termed ______.

Accepted Answer

A)   antisepsis
B)   disinfection
C)   sterilization
D)   decontamination
E)   sanitizationF) A) and C)
G) A) and B)

Question 14

Which of the following is not a factor that affects germicidal activity?

Accepted Answer

A)   Material being treated
B)   Length of exposure
C)   Strength of the germicide
D)   Microorganism being treated
E)   All of the choices are factors.F) B) and D)
G) C) and E)

Question 15

_____ is a control method that removes microorganisms rather than inhibiting or killing them.

Accepted Answer

A)   Disinfection
B)   Boiling
C)   Filtration
D)   Sterilization
E)   RadiationF) D) and E)
G) A) and D)

Question 16

The difference between thermal death time and thermal death point in microbicidal activity is that _______.

Accepted Answer

A)   thermal death time is the shortest amount of time required to kill an organism at a 100oC, whereas thermal death temperature is the lowest temperature required to kill an organismIn 10 minutes
B)   thermal death time is the shortest amount of time required to kill an organism at a speciﬁc temperature, whereas thermal death temperature is the lowest temperature required to killAn organism in 10 minutes
C)   thermal death time is the greatest amount of time required to kill an organism at a speciﬁc temperature, whereas thermal death temperature is the lowest temperature required to killAn organism in 10 minutes
D)   thermal death time is the shortest amount of time required to kill an organism at a speciﬁc temperature, whereas thermal death temperature is the lowest temperature required to killE) None of the above
F) A) and B)

Question 17

Soaps and detergents are very effective as sterilants.

Accepted Answer

A) True 
 B)False

Question 18

Which of the following is a disadvantage of dry heat methods such as using a Bunsen burner to incinerate microbes from an inoculating loop and the use of a hot air oven?

Accepted Answer

A)   Use of an open ﬂame can be dangerous and hot air ovens take much longer to sterilize than autoclaves.
B)   Bunsen burners and hot air ovens only reach a maximum of 100oC so they are not effective against endospores.
C)   Neither of these methods actually sterilize; they only kill vegetative cells.
D)   Bunsen burners take too long to sterilize and hot air ovens are hazardous for the operators.E) All of the above
F) A) and B)

Question 19

The minimum sterilizing conditions in a steam autoclave are _______.

Accepted Answer

A)   71.6°C for 15 seconds
B)   63°C for 30 minutes
C)   100°C for 30 minutes
D)   160°C for 2 hours
E)   121°C at 15 psi for 15 minutesF) B) and C)
G) C) and E)

Question 20

Which of the following types of control agents would be used to achieve sterility?

Accepted Answer

A)   Virucide
B)   Bactericide
C)   Germicide
D)   Sporicide
E)   FungicideF) B) and C)
G) A) and E)

Exam 11: Physical and Chemical Control of Microbes

Alcohols _______.

Correct AnswerverifiedShow Answer

_____ is a halogen used in gaseous and liquid form for large scale disinfection of drinking water and sewage.

Correct AnswerverifiedShow Answer

The difference between thermal death time and thermal death point in microbicidal activity is that _______.

Correct AnswerverifiedShow Answer

Which of the following is not a factor that affects germicidal activity?

Correct AnswerverifiedShow Answer

Which of the following is a disadvantage of dry heat methods such as using a Bunsen burner to incinerate microbes from an inoculating loop and the use of a hot air oven?

Correct AnswerverifiedShow Answer

The method of removing vegetative microbial life forms and debris to reduce contamination to safe levels is termed ______.

Correct AnswerverifiedShow Answer

Intermittent sterilization, which uses 3 days of lower temperature steam for short periods of time, is also called ______.

Correct AnswerverifiedShow Answer

Organisms in the genus Pseudomonas are resistant to quats.

Correct AnswerverifiedShow Answer

A method for sterilizing milk, called _____ treatment, uses 134°C for 1 to 2 seconds.

Correct AnswerverifiedShow Answer

The presence of organic matter such as saliva and pus can interfere with the actions of disinfectants.

Correct AnswerverifiedShow Answer

Endospores can be inactivated by _______.

Correct AnswerverifiedShow Answer

Surfactants work by _______.

Correct AnswerverifiedShow Answer

The minimum sterilizing conditions in a steam autoclave are _______.

Correct AnswerverifiedShow Answer

Chlorine and ethylene oxide are sterilizing gases.

Correct AnswerverifiedShow Answer

_____ is a control method that removes microorganisms rather than inhibiting or killing them.

Correct AnswerverifiedShow Answer

Continued widespread use of the phenolic compound triclosan is advantageous in that it is important to remove as many organisms as possible from our hands and there is little risk of resistant organisms evolving.

Correct AnswerverifiedShow Answer

Which of the following types of control agents would be used to achieve sterility?

Correct AnswerverifiedShow Answer

Which of the following represents microbial control by osmotic pressure?

Correct AnswerverifiedShow Answer

Soaps and detergents are very effective as sterilants.

Correct AnswerverifiedShow Answer

When a control agent targets the metabolic processes of microbial cells, active younger cells typically die more rapidly than older cells.

Correct AnswerverifiedShow Answer

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